Research project CRISPR: tailored for vegetatively propagated plants
General introduction
The new genomic techniques (NGTs), including CRISPR/Cas, make it possible to introduce desirable traits in a highly efficient, targeted, and rapid manner. NGTs hold great potential for the development of new, adapted cultivars—particularly in vegetatively propagated ornamental and fruit crops—with traits such as altered morphology, improved shelf life, and increased disease tolerance. The Flemish breeding sector for ornamental and fruit crops is eager to implement NGTs in their operations, as these techniques can significantly shorten the breeding time. Exactly the same useful modifications can be achieved without NGT, only it is years more work, more trial and error and more expensive. The overall aim of this project is therefore to implement the possibilities and applications of CRISPR/Cas-based technologies in vegetatively propagated ornamental plants and fruit crops.
Research approach
ILVO develops CRISPR/Cas protocols for three case studies: (1) modificatin of flower color in Petunia, (2) reduced ethylene sensitivity in Pelargonium, and (3) increased resistance to powdery mildew in apple. Each of these crops differs in the amount of available genomic knowledge, so different strategies will need to be developed for identifying target genes, designing gRNAs, performing in vitro transformation and regeneration, and screening for potential mutants. In addition, methods will be established and validated for phenotyping plant morphology (Petunia), ethylene sensitivity (Pelargonium), and disease resistance (apple). All the knowledge generated will contribute to the development of a feasibility scan to support the application of CRISPR/Cas by target companies in their own crops.
Relevance/Valorization
The project will lead to a transition in which Flemish breeding companies can use CRISPR/Cas as a complement to traditional breeding techniques. This will enable breeding programs to progress more rapidly and create new cultivars tailored to the needs of consumers and society. At the time of the start of the project CRISPRGEKNIPT, a European-level decision is currently being made on whether or not to implement new legislation to allow NGT products on the market. If this legislation is approved, Flemish breeding companies will be able to fully utilize CRISPR/Cas technology to accelerate the development and commercialization of innovative cultivars, helping them remain competitive both within Europe and globally.
Financing
VLAIO - Agentschap Innoveren & Ondernemen
